Other factors including the cost of the tracer uc glucose is. Metabolic flux analysis using clabeled substrates has become an important tool. Finally we discuss applications using metabolic flux analysis to. Fba and c metabolic flux analysis mfa, are widely used to predict or. A limitation of cmfa is that it is based on the assumption of. Results we have developed openflux as a user friendly, yet flexible software application for small and large scale c metabolic flux analysis. Metabolic flux analysis mfa plays a key role in systems biology because intracellular fluxes, i. It thus is an important complement to the detection of global transcript, protein, and metabolite concentrationsthe network components. This is a pdf file of an unedited manuscript that has been accepted for publication. Isotopebased metabolic flux analysis is one of the emerging technologies applied to system level metabolic phenotype characterization in metabolic engineering. Here, we used fluxbased modeling approaches to improve yields of. Stable isotope, and in particular cbased flux analysis, is the exclusive approach to experimentally quantify the integrated responses of metabolic networks.
Metabolic flux analysis based on stable isotope experiments is a quantitative method to assess gene, protein, and metabolite interactions within metabolic networks. Appendix v of this publication is not included in the pdf version. Currently, c metabolic flux analysis cmfa is the preferred tool for quantitative characterization of metabolic phenotypes in microbial 32,33,34 and mammalian cells 3,4,35,36,37,38. Highresolution c metabolic flux analysis nature protocols.